IN SITU VASCULAR TISSUE REMODELING USING BIODEGRADABLE TUBULAR SCAFFOLDS WITH INCORPORATED GROWTH FACTORS AND CHEMOATTRACTANT MOLECULES

L. V. Antonova; V. V. Sevostyanova; A. V. Mironov; E. O. Krivkina; E. A. Velikanova; V. G. Matveeva; T. V. Glushkova; Y. L. Elgudin; L. S. Barbarash

Статья

IN SITU VASCULAR TISSUE REMODELING USING BIODEGRADABLE TUBULAR SCAFFOLDS WITH INCORPORATED GROWTH FACTORS AND CHEMOATTRACTANT MOLECULES

L. V. Antonova, V. V. Sevostyanova, A. V. Mironov, E. O. Krivkina, E. A. Velikanova, V. G. Matveeva, T. V. Glushkova, Y. L. Elgudin, L. S. Barbarash

2018

https://doi.org/10.17802/2306-1278-2018-7-2-25-36

Background Currently, the search for the bioactive molecules capable of promoting formation of the vascular tissue is still ongoing. We have previously demonstrated that incorporation of the growth factors and chemoattractant molecules into the biodegradable tubular scaffolds can increase their primary patency upon the implantation into rat abdominal aorta. However, further studies are required to investigate tissue remodeling using functionalized vascular grafts with the same diameter as a replaced native vessel. Aim To investigate the specific aspects of de novo vascular tissue formation and calcification employing rat abdominal aorta interposition model and vascular grafts with 1.5 mm diameter with incorporated vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and stromal cell-derived factor (SDF)-1α. Methods Tubular grafts with a diameter of 1.5 mm were blended of poly(3-hydroxybutyrateco-3-hydroxyvalerate) and poly(ε-caprolactone) (PHBV/PCL). Grafts without growth factors were fabricated using standard electrospinning technique whilst grafts with incorporated growth factors were prepared utilizing emulsion electrospinning. VEGF was incorporated into the inner third, whereas bFGF and SDF-1α were incorporated into the outer two-thirds of the graft. Grafts were implanted into the abdominal aortas of Wistar rats for 1, 3, 6, and 12 months following scanning electron microscopy along with histological and immunofluorescent examination. Results Primary patency of the grafts with VEGF, bFGF, and SDF-1α reached 93% indicative of structural integrity of the vascular tissue. Neither signs of inflammation nor severe calcification was detected. Conclusion As in 2 mm diameter vascular grafts, incorporation of bioactive factors into 1.5 mm diameter grafts increased their long-term primary patency and improved vascular tissue formation in comparison with non-modified grafts.

Antonova L. V., Sevostyanova V. V., Mironov A. V., Krivkina E. O., Velikanova E. A., Matveeva V. G., Glushkova T. V., Elgudin Ya. L., Barbarash L. S. IN SITU VASCULAR TISSUE REMODELING USING BIODEGRADABLE TUBULAR SCAFFOLDS WITH INCORPORATED GROWTH FACTORS AND CHEMOATTRACTANT MOLECULES. Комплексные проблемы сердечно-сосудистых заболеваний. 2018;7(2):25-36. https://doi.org/10.17802/2306-1278-2018-7-2-25-36

Цитирование

Список литературы

1. Tara S., Rocco K.A., Hibino N., Sugiura T., Kurobe H., Breuer C.K. et al. Vessel bioengineering. Circ J. 2014; 78(1): 12–9.

2. Antonova L.V., Seifalian A.M., Kutikhin A.G., Sevostyanova V.V., Matveeva V.G., Velikanova E.A. et al. Conjugation with RGD peptides and incorporation of vascular endothelial growth factor are equally efﬁcient for biofunctionalization of tissue-engineered vascular grafts./ International Journal of Molecular Sciences 2016; 17(11): 1920. doi:10.3390/ijms17111920.

3. Sevostyanova V.V., Matveeva V.G., Antonova L.V., Velikanova E.A., Shabaev A.R., Senokosova E.A. et al. Constructing a Blood Vessel on the Porous Scaffold Modiﬁed with Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor. AIP Conference Proceedings. 2016; 1783(1): 020204. doi: 10.1063/1.4966498.

4. Antonova L.V., Krivkina E.O., Sevostyanova V.V., Velikanova E.A., Matveeva V.G., Mironov A.V. et al. Efﬁciency of using bioactive molecules in creation of functional biodegradated vascular grafts of small diameter. Siberian Medical Review. 2017;(6): 85-93. DOI: 10.20333/2500136-2017-6-85-9 (in Russian).

5. Glushkova T.V., Sevostyanova V.V., Antonova L.V., Klyshnikov K.Y., Ovcharenko E.A., Sergeeva E.A. et al. Biomechanical remodeling of biodegradable small-diameter vascular grafts in situ. Russian Journal of Transplantology and Artiﬁcial Organs. 2016; 18(2):99-109. (in Russian).

6. Nasonova M.V., Shishkova D.K., Antonova L.V., Sevostyanova V.V., Kudryavtseva Y.A., Barbarash O.L. et al. Subcutaneous Implantation of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and Poly(ε-caprolactone) Scaffolds Modiﬁed with Growth Factors. Sovremennye tehnologii v medicine. 2017; 9(2): С 7-18. (in Russian)

7. Matveeva V.G., Antonova L.V., Sevost’janova V.V., Velikanova E.A., Krivkina E.O., Glushkova T.V. et al. Modiﬁcation by RGD-peptides оf vascular grafts of small diameter from polyparolactone: experimental study results. Kompleksnye problemy serdechno-sosudistyh zabolevanij. 2017. (3): 13-24 (in Russian).

8. Ren X., Feng Y., Guo J., Wang H., Li Q., Yang J. et al. Surface modiﬁcation and endothelialization of biomaterials as potential scaffolds for vascular tissue engineering applications. Chem Soc Rev. 2015; 44(15): 5680-742.

9. Kurobe H., Maxﬁeld M.W., Tara S., Rocco K.A., Bagi P.S., Yi T. et al. Development of small diameter nanoﬁber tissue engineered arterial grafts. PLoS One. 2015; 10(4): e0120328.

10. Chong D.S., Lindsey B., Dalby M.J., Gadegaard N., Seifalian A.M., Hamilton G. Luminal surface engineering, ‘micro and nanopatterning’: potential for self endothelialising vascular grafts? Eur J Vasc Endovasc Surg. 2014; 47(5): 566-76.

11. d’Arcy J.L., Prendergast B.D., Chambers J.B., Ray S.G., Bridgewater B. et al. Valvular heart disease: the next cardiac epidemic. Heart 2011; 97(2): 91-93. doi: 10.1136/ hrt.2010.205096.

12. Farzaneh-Far A., Proudfoot D., Shanahan C. Weissberg P.L. Vascular and valvar calciﬁcation: recent advances. Heart 2001; 85: 13–17. doi: 10.1136/heart.85.1.13.

13. Schoen F.J., Levy R.J. Calciﬁcation of Tissue Heart Valve Substitutes: Progress Toward Understanding and Prevention. Ann Thorac Surg 2005; 79: 1072–80. doi: 10.1016/j. athoracsur.2004.06.033.

14. New S.E., Aikawa E. Role of extracellular vesicles in de novo mineralization: an additional novel mechanism of cardiovascular calciﬁcation. Arterioscler Thromb Vasc Biol 2013; 33(8): 1753–8. doi.org/10.1161/CIRCRESAHA.110.234146.

15. Goettsch C., Hutcheson J.D., Aikawa E. MicroRNA in cardiovascularcalciﬁcation: focus on targets and extracellular vesicle deliverymechanisms. Circ Res 2013; 112(7): 1073–84.

16. Leopold J.A. Vascular calciﬁcation: mechanisms of vascular smooth musclecell calciﬁcation. Trends Cardiovasc Med. 2015; 25(4): 267-74. doi: 10.1016/j.tcm.2014.10.021.

17. Bujan J., Bellh J.M., Sabater C., Jurado F., Garcia-Honduvilla N., Dominguez B. et al. Modiﬁcations induced by atherogenic diet in the capacity of the arterial wall in rats to respond to surgical insult. Atherosclerosis1996; 122(2): 141–52.

18. Bostrom K.I., Rajamannan N.M., Towler D.A. The regulation of valvular andvascular sclerosis by osteogenic morphogens. Circ Res 2011; 109(5): 564–577. doi.org/10.1161/ CIRCRESAHA.110.234278.

19. Tintut Y., Patel J., Parhami F., Demer L.L. Tumor necrosis factor-alpha promotes in vitro calciﬁcation ofvascular cells via the cAMP pathway. Circulation 2000; 102(21): 2636–42. doi.org/10.1161/01.CIR.102.21.2636.

20. Cote N., Mahmut A., Bosse Y., Couture C., Pagé S., Trahan S. et al. Inﬂammation is associated with the remodeling of calciﬁcaortic valve disease. Inﬂammation 2013; 36(3): 573– 581. doi.org/10.1007/s10753-012-9579-6.

21. Fadini G.P., Rattazzi M., Matsumoto T., Asahara T., Khosla S. Emerging role of circulating calcifying cells in thebone-vascular axis. Circulation 2012; 125(22): 2772–81.doi. org/10.1161/CIRCULATIONAHA.112.090860.

22. Gossl M., Khosla S., Zhang X., Higano N., Jordan K.L., Loefﬂer D. et al. A. Role of circulating osteogenic progenitor cells in calciﬁc aorticstenosis. J. Am Coll Cardiol 2012; 60(19): 1945–1953. doi.org/10.1016/j.jacc.2012.07.042.

23. Cottignoli V., Cavarretta E., Salvador L., Valfré C., Maras A. Morphological and Chemical Study of Pathological Deposits in Human Aortic and Mitral Valve Stenosis: A Biomineralogical Contribution. Patholog Res Int. 2015; 2015: 342984. doi: 10.1155/2015/342984.

24. Pettenazzo E., Deiwick M., Thiene G., Molin G., Glasmacher B., Martignago F. et al. Dynamic in vitro calciﬁcation of bioprosthetic porcine valves evidence of apatite crystallization. The Journal of Thoracic and Cardiovascular Surgery 2001; 121(3): 500-509. doi: 10.1067/mtc.2001.112464.

25. Kudrjavceva Ju.A., Nasonova M.V., Akent’eva T.N., Burago A.Ju., Zhuravleva I.Ju. The role of suture material in the calciﬁcation of cardiovascular bioprosthesis. Kompleksnye problemy serdechno-sosudistyh zabolevanij. 2013; 4: 22-27 (in Russian).

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Информация

Автор

L. V. Antonova Research Institute for Complex Issues of Cardiovascular Diseases
V. V. Sevostyanova Research Institute for Complex Issues of Cardiovascular Diseases
A. V. Mironov Research Institute for Complex Issues of Cardiovascular Diseases
E. O. Krivkina Research Institute for Complex Issues of Cardiovascular Diseases
E. A. Velikanova Research Institute for Complex Issues of Cardiovascular Diseases
V. G. Matveeva Research Institute for Complex Issues of Cardiovascular Diseases
T. V. Glushkova Research Institute for Complex Issues of Cardiovascular Diseases
Y. L. Elgudin Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center
L. S. Barbarash Research Institute for Complex Issues of Cardiovascular Diseases

Название журнала

Комплексные проблемы сердечно-сосудистых заболеваний

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Статья