Статья
АНАЛИЗ ВЛИЯНИЯ ВОЗДЕЙСТВИЯ ЦИТРАТА МАГНИЯ И ПРОТЕИНОГЕННЫХ АМИНОКИСЛОТ С РАЗВЕТВЛЕННЫМ СТРОЕНИЕМ АЛИФАТИЧЕСКОЙ БОКОВОЙ ЦЕПИ НА КОНЦЕНТРАЦИЮ КАЛЬЦИПРОТЕИНОВЫХ ЧАСТИЦ В ЦИРКУЛИРУЮЩЕЙ КРОВИ
Основные положенияПероральное употребление протеиногенных аминокислот с разветвленным строением алифатической боковой цепи (лейцина, изолейцина и валина) и цитрата магния гиперлипидемическими мышами не оказывает влияния на концентрацию кальципротеиновых частиц (КПЧ) в сыворотке крови.Добавление цитрата магния к перенасыщенной ионами кальция и фосфора сыворотке крови пациентов с инфарктом миокарда с подъемом сегмента ST приводит к статистически значимому снижению концентрации КПЧ.Полученные результаты позволяют предположить потенциальную полезность цитрата магния для снижения циркуляции КПЧ в крови после агрегации ими избыточного кальция и фосфора. Цель. Оценить воздействие протеиногенных аминокислот с разветвленным строением алифатической боковой цепи (лейцина, изолейцина и валина – BCAA) и цитрата магния на концентрацию кальципротеиновых частиц (КПЧ) в крови гиперлипидемических (ApoE-нокаутных) мышей, а также в крови пациентов с инфарктом миокарда с подъемом сегмента ST (ИМпST) при добавлении цитрата магния ex vivo.Материал и методы. В исследование было включено 270 ApoE-нокаутных мышей (135 самцов и 135 самок), получавших BCAA (55 мг/сут, что эквивалентно 13,5 г/сут для человека) или цитрат магния (1,85 мг/сут, что эквивалентно 450 мг/сут для человека) с 1-месячного, 3-месячного или 6-месячного возраста (по 90 мышей в каждой группе, из них по 45 самцов и 45 самок). В 12-месячном возрасте всех животных выводили из эксперимента и проводили проточно-цитометрический анализ концентрации КПЧ в сыворотке крови при помощи флюоресцентно меченного бисфосфоната. Также в исследование было включено 27 пациентов с ИМпST, у которых получали сыворотку крови с добавлением к ней CaCl2 и Na2HPO4*12H2O для создания минерального стресса и последующим добавлением цитрата магния для оценки его способности снижать формирование КПЧ.Результаты. Пероральный прием BCAA и цитрата магния ApoE-нокаутными мышами не влиял на концентрацию КПЧ в сыворотке крови. В то же время добавление цитрата магния к перенасыщенной ионами кальция и фосфора сыворотке крови пациентов с ИМпST приводило к снижению концентрации КПЧ в 74% случаев (20/27), при этом в 81% случаев (22/27) концентрация КПЧ снижалась более, чем в сыворотке без перенасыщения ионами кальция и фосфора.Заключение. Полученные данные позволяют предположить терапевтический эффект цитрата магния, обладающего хелатирующим действием и растворяющим циркулирующие в крови КПЧ.
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